SPART Knockout cell line (Hep G2)

ENCN
location： Home > Products > KO Cell Lines > SPART Knockout cell line (Hep G2)

SPART Knockout cell line (Hep G2) Order

SPART Knockout cell line (Hep G2)
Catalog#
YKO-HT7305
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SPART Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SPART Knockout cell line (Hep G2)
Species
Human
Gene Name
SPART
Gene ID
23111
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript SPART-202)
Validation: PCR+Sanger Sequencing
SPART Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SPART gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SPART gene knockout SPART Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SPART Knockout cell line (Hep G2)
SPART Knockout cell line (Hep G2)
Catalog#
YKO-HT7305
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • SPART Knockout cell line (HeLa)

    SPART Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: SPART
    Gene ID: 23111
    Size: 1*10^6
    Catalog#: YKO-XN48612
    YKO-XN48612(HeLa)
  • SPART Knockout cell line (HEK293)

    SPART Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: SPART
    Gene ID: 23111
    Size: 1*10^6
    Catalog#: YKO-XN48610
    YKO-XN48610(HEK293)
  • SPART Knockout cell line (A549)

    SPART Knockout cell line (A549)
      inquiry   More details

    Organism: Human
    Gene Name: SPART
    Gene ID: 23111
    Size: 1*10^6
    Catalog#: YKO-XN48609
    YKO-XN48609(A549)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • SPART Knockout cell line (HeLa)

    SPART Knockout cell line (HeLa)

    Organism: Human
    Gene Name: SPART
    Gene ID: 23111
    Size: 1*10^6
    Catalog#: YKO-XN48612
      inquiry   More details
  • SPART Knockout cell line (HEK293)

    SPART Knockout cell line (HEK293)

    Organism: Human
    Gene Name: SPART
    Gene ID: 23111
    Size: 1*10^6
    Catalog#: YKO-XN48610
      inquiry   More details
  • SPART Knockout cell line (A549)

    SPART Knockout cell line (A549)

    Organism: Human
    Gene Name: SPART
    Gene ID: 23111
    Size: 1*10^6
    Catalog#: YKO-XN48609
      inquiry   More details
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now